EFFICIENT 2D-SEPARATION TECHNIQUE THROUGH COUPLING OF IC AND CE FOR HIGH THROUGHPUT MS ANALYSIS
High-quality mass spectrometry analysis requires a high-resolution pre-separation of the sample. Therefore, chromatographic separation techniques like Ion Chromatography (IC) and Capillary Electrophoresis (CE) are widely used. However, these one-dimensional separation methods are limited in terms of resolution, since some species behave similar within one separation technique.
To reach a higher resolution, different techniques can be combined. The present invention offers a solution for the combination of IC and CE, based on the coupling of the IC- and CE-apparatus through a modulator. The separation consists of the following steps:
1. Separation through IC
2. Transfer of the sample from IC- to CE-apparatus through the modulator
3. Separation through the CE-capillary
4. Mass spectrometry analysis
The device and method developed in the frame of the invention allow for an improved separation of ionic species, providing increased peak capacity and better cost-efficiency. Small sample volumes and short run-times ensure high throughput analysis compared to available methods.
Setup of the two-dimensional separation system consisting of Ion Chromatography (IC), a modulator and Capillary Electrophoresis (CE) connected to a mass spectrometer (MS) with an exemplary measurement result.
COMMERCIAL OPPOR TUNITIES
This method is particularly suited for the investigation of small molecules such as nucleic acids, amino acids and arsenic compounds. Therefore, this invention can be applied in the field of diagnosis and monitoring of human decease’s as well as toxicology and forensic chemistry.
A prototype is established and fully operational.
1) Anal. Chem. 87, 3134-3138 (2016)
2) EP 3 134 725 A1; US 2017 0030860 A1
3) Anal. Bioanal. Chem. 410, 6321-6330 (2018)