HYDRODABCYL: A HYDROPHILIC DARK QUENCHER
Dabcyl is one of the most frequently used fluorescence quenchers. However, the very poor solubility in aqueous solutions due to its hydrophobic properties limits its use in biological systems where the natural solvent is water. The present invention provides a new dark quencher called Hydrodabcyl, which represents the hydrophilic alternative to Dabcyl. The spectroscopic properties of Hydrodabcyl are similar to that of Dabcyl.
The present invention provides a convenient and reliable new dark quencher for FRET-based applications. As a water-soluble agent, Hydrodabcyl can be used in a large variety of biological and biochemical systems and is an improved alternative to the very popular Dabcyl for the design of fluorogenic probes. Due to its non-immunogenic and non-toxic properties Hydrodabcyl is highly suitable for in vitro as well as in vivo applications.
Advantages of Hydrodabcyl:
- very well soluble in aqueous solution over the whole biologically relevant pH range
- dark quencher: quenching range 350-550 nm
- no additional charges
- substantially easier to handle than dabcyl
- higher stability to light exposure in comparison to dabcyl
- non-toxic and non-immunogenic
Proof of concept, ready to use.
Anal. Chem. 89, 22, 11893-11897