METHOD AND DEVICE FOR DETERMINING THE PRESENCE OF AN ANALYTE BY MEANS OF SMALL MAGNETIC PARTICLES
Magnetic nanoparticles, especially with a functional coating, are used now for more than 10 years for bioanalytical methods or as a contrast medium for magnetic resonance imaging in clinical and pre-clinical trials. In this respect a possible application scenario is the labelling of stem cells with magnetic nanoparticles to target and image them. The commercial availability and the particular characteristics of these particles open up new possibilities for bioanalytical and diagnostic methods.
The proposed invention allows the specific detection and quantification of magnetic nanoparticles, especially dispersed in liquid media. The particle surface can be modified with certain linker molecules and binding sites, the so called functionalization layer. Thereby different analytes, for example antigens, viruses or certain molecules, can bind on the surface. With the proposed technology the respective analytes can be detected by measuring changes in the physical characteristics of the nanoparticles. The technology allows the construction of bio-analytical sensors which have a comparable sensitivity to conventional optical read-out techniques, however combined with a high level of robustness and low material consumption. Moreover also optically not accessible samples can be characterized, which is of particular importance for in-vivo diagnostics. Additionally a parallel detection of several different analytes in the same test sample can be conducted, which reduces the costs in comparison to conventional technologies dramatically.
- Molecular „fingerprint“ analysis of blood samples – by detecting characteristic distribution of different types of molecules possible diseases can be diagnosed
- Early detection of cancer
- Chemical reactions or other liquid samples can be monitored
- Rapid diagnosis or analysis device for blood or tissue samples. These devices can find application in hospitals, medical practices, analysis laboratories or in research institutes
- High level of robustness in comparison to conventional optical read-out techniques
- „Low Cost Equipment“ for point-of-care diagnostics can be realized, for example for the tuberculosis detection in developing countries.
First measurement results were obtained. A prototype is completed.
(1) EP2553455 A1 / US2013157256 A1